KMID : 0617319950050010129
|
|
Journal of Pharmacetical Sceiences Ewha Womans University 1995 Volume.5 No. 1 p.129 ~ p.133
|
|
Simple and sensitive laser-induced fluorescence detection for capillary electrophoresis and its application to protein separation
|
|
Lim Heung-Bin
Lee Jong-Jin Lee Kong-Joo
|
|
Abstract
|
|
|
A low-cost and highly sensitive laser-induced fluorescence detector for on-column detection systems was constructed and its applications were demonstrated in protein separation by capillary electrophoresis. The limit of detection (LOD) at a signal-to-noise ratio of 2 for Lissamine 20 was about 1.4 ¡¿ 10 exp(-21) mole at 10 nL sample injection and the relative standard deviation (RSD) for 1.5 ¡¿ 10 exp(-11) M of Lissamine 20 solution was about 15%. These values are comparable to or even better than those reported using a similar on-column detection system. This sensitive LIF detection was applied for the study of tryptic digestion of insulin and for urinary protein profiling.
|
|
KEYWORD
|
|
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|